spy650 fastact x (Cytoskeleton Inc)

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Cytoskeleton Inc spy650 fastact x
Spy650 Fastact X, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spy650+fastact+x/SPY650-FastAct/bio_rxiv__64898__2026__04__27__721156-173-0-1
Average 95 stars, based on 36 article reviews

spy650 fastact x - by Bioz Stars, 2026-09

95/100 stars

Images

Imaging:

Article Title: Mechanically-induced Septin Networks Protect Nuclear Integrity
Article Snippet: Hoechst (H3570; Invitrogen; live imaging 1:5000 for 10 min). .. SPY650-FastAct_X (CY-SC502; Cytoskeleton; live imaging 1:1000). .. Janelia Fluor JFX554 HaloTag Ligand (HT103A;Promega; live imaging 10-50nM) Janelia Fluor 646 HaloTag Ligand (HT106A;Promega; live imaging 10-50nM)

Cell Culture:

Article Title: EPS8 dampens the growth dynamics and prolongs the lifetime of actin-based protrusions
Article Snippet: .. SPY650-FastAct_X (Cytoskeleton #CY-CS502) was added to cell culture media at 1:1000 ( ) or 1:2000 ( ); cells were then incubated for 30 min - 1 hr before labeling media was removed and replaced with fresh media. .. For cell staining: Cells were rinsed in prewarmed PBS and fixed by incubating in prewarmed 4% paraformaldehyde (Electron Microscopy Services #15710) for 15 min at 37° C. Cells were then rinsed 3x for 5 min with PBS and permeabilized in 0.1% Triton X-100 (Sigma #T8787) for 15 min at RT.

Incubation:

Labeling:

Article Title: Septins buffer actomyosin forces to protect the nucleus from genotoxic mechanical stress
Article Snippet: The following fluorescent antibodies were used for immunodetection of primary antibodies in immunofluorescence experiments: Alexa Fluor 488 AffiniPure donkey anti-rat IgG (H+L) (Jackson ImmunoResearch, 712-545-153), Alexa Fluor 488 AffiniPure F(ab)2 donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, 712-545-153), AlexaFluor 488 AffiniPure F(ab)2 donkey anti-mouse IgG (H+L) (Jackson ImmunoResearch, 715-546-150), Alexa Fluor 547 AffiniPure F(ab)2 donkey anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, 711-546-152). .. Actin was labeled in fixed cells with AF488 Phalloidin (AAT Bioquest, 23153) and Phalloidin-iFluor 647 (Abcam, ab176759), and in live cells with SPY555-Actin (Cytoskeleton, CY-SC202), SPY555-FastAct (Cytoskeleton, CY-SC205) or SPY650-FastAct_X (Cytoskeleton, CY-SC502). .. Cell nuclei were stained with DAPI (1 μg/ml; ThermoFisher Scientific) in fixed cells and the Hoechst 3342 (Invitrogen) in live cells.

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